DNA

Part:BBa_K4051021:Design

Designed by: Clodagh Healy   Group: iGEM21_MTU-CORK   (2021-10-11)


Cld GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 452
    Illegal BsaI.rc site found at 734
    Illegal SapI.rc site found at 614


Design Notes

codon optimised for E.coli


Source

Generic Histag used

Generic Secretion Signal from Gaussian luciferase Dechloromonas (Azospira) suillum locus Dsui_0145

Genbank Accession: CP003153.1


References